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The use of immobilized Candida rugosa on Amberlite IRC-50 to treat rich-lipids medium and to reduce pollution in high oil and grease wastewater

Congress: 2008
Author(s): Emilienne RASOANANDRASANA, Martial Zozime RASOLONJATOVO
Department of Environment and Wastes, Faculty of Sciences, Majunga University, P.O. Box 652, Majunga, 401, MADAGASCAR; *Corresponding author: rasoanandrasana@yahoo.fr
AbstractAbstract Hydrolysis of lipids and treatment of wastewaters with high concentration of oil and grease were tested using Candida rugosa lipase immobilized on Amberlite IRC-50. The enzyme was adsorbed completely on Amberlite IRC-50 by the dried process. Lipase from Candida rugosa immobilized on Amberlite IRC-50 showed a high specific activity of 582 U/mg protein where 1 U is the amount of lipase enzyme required to produce 1 μmol of fatty acid per minute. The lipase was less sensitive to pH (4 9) and temperature (20-50C) after immobilization. The immobilized enzyme hydrolysed more than 60% of the oils and grease from the food industry wastewater. During the 7-day experiment, after the O&G hydrolysis, approximately 80% of the enzyme activity remained in the beads. The hydrolysis of olive oil by the immobilized enzyme before and after O&G hydrolysis confirmed the stability of the immobilizate This study shows that Candida rugosa immobilized on Amberlite IRC-50 by dried process can be used in the pre-treatment of high oil and grease wastewater.
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